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2D- and continuous wave (CW) Doppler echocardiography with standard axis views were performed by one highly experienced cardiologist using a Sonos 7500 (Philips Medizin Systeme GmbH, Hamburg, Germany) ultrasound machine according to the recommendations of the American Society of Echocardiography [ 10, 18, 22].
When Ms. Marshall mentions an upcoming date, Ms. Hamburg, a perpetual advice machine, issues a warning: "Now remember, he's 15 years older than you are.
The PCR reactions were performed with a Mastercycler machine (Eppendorf AG, Hamburg, Germany).
Microsatellites were amplified with a Mastercycle Gradient machine (Eppendorf, Hamburg, Germany) using the Qiagen® Multiplex PCR Kit (Qiagen, Hilden, Germany).
For sequencing analysis, PCR products were purified in 96-format Acro Prep Filter Plates (Pall Life Sciences, Ann Arbor, MI, USA) using the Perfect Vac Manifold vacuum machine (Eppendorf AG, Hamburg, Germany).
Real-time PCR was performed by using the SYBR® Premix Ex Taq™ II (TaKaRa, Dalian, China) on a Mastercycler® ep realplex machine (Eppendorf, Hamburg, Germany).
The gene expressions were quantified by rRT-PCR, using an iQ SYBR Green Supermix (Bio-Rad, USA) and a gradient cycler machine (Eppendorf, Hamburg, Germany).
Animals or cultured spermatogonia received a dose of 2.0 Gy (unless otherwise indicated) irradiation at a rate of 1.04 1.68 Gy/min using a 320-kVp Philips industrial X-ray machine (GMBH, Hamburg, Germany).
Cyt b sequences were amplified with a Mastercycle Gradient machine (Eppendorf, Hamburg, Germany) using a Maxima Hot Start PCR Master Mix (Fermentas GmbH, St. Leon-Rot, Germany) and zcyt-1F (5′-taatggcaagcctccgaaa-3′) and zcyt-2R (5′-ctgagctactaatgcaggatca-3′) as forward and reverse primers, respectively.
Furthermore, we have demonstrated repeatable and accurate whole smoke dilution (ranging from 1 2 1 4,000 smoke:air, volume:volume) and delivery of cigarette smoke using a commercially available Borgwaldt RM20S smoking machine (Borgwaldt-kc, Hamburg, Germany) [2, 6] and successful applications in vitro[7, 9, 11].
PHVHF was performed using a multifiltrate CRRT machine (Fresenious Medical Care, Bad Hamburg, Germany).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com