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The result is a compromise — richer nations have to transition faster than poorer ones, but poorer ones must halt using HFCs in time, too.
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The reaction was halted using 50 mM Tris/HCl pH 7.5.
The assay was then halted using 25 μl of Beadlyte Stop Solution.
The DAB reaction was halted using PBS, followed by three 10 min PBS rinses.
Transposition reactions were halted using QG buffer (Qiagen) and fragmented cDNA was purified on AMPure XP beads.
After fixation, the crosslinking reaction was halted using 0.125 M glycine for 5 minutes while rocking at room temperature.
However this approach can induce a stress response within the cell and further transcription must be halted using actinomycin D. This combination was used by Bloethner et al. (2008) in an approach called Gene Identification by NMD Inhibition.
Outlier analysis was conducted for F6-generation individuals (i.e. first generation after the selection was halted) using the Fdist method (Beaumont and Nichols 1996) implemented in software LOSITAN – Selection Workbench (Antao et al. 2008).
AdAge has joined the growing cry to halt the use of great apes in advertising, editorializing that despite their comic potential, "It's time to stop using them for the sake of selling product".
The Army memo, first published at Small UAS News and dated August 2, said that "due to increased awareness of cyber vulnerabilities associated with DJI products, it is directed that the U.S. Army halt use of all DJI products".
But it uses RD-180 engines, which are designed and built in Russia, and there's a Congressionally-mandated deadline to halt use of the Russian-made RD-180s for military launches by December 31 , 2022
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