Sentence examples for haloalkane from inspiring English sources
noun
Any alkane in which one or more hydrogen atoms has been replaced by that of a halogen
A method for the detection of haloalkane conversion to the corresponding alcohols by haloalkane dehalogenases is described.
Rhodococcus haloalkane dehalogenase (DhaA) removes halides from aliphatic hydrocarbons by a nucleophilic displacement mechanism.
For the detection of haloalkanes or haloalkane dehalogenase, respectively, activity via a multistep bioassay, the haloalkane dehalogenase DhaA from R. erythropolis was used as a model enzyme.
Furthermore, the established multistage enzyme reaction can be considered as a modular system for haloalkane detection.
The KEGG database suggested this gene is dhaA haloalkane dehalogenase, and has xenobiotics biodegradation and metabolism function.
c The Halo-tag derives from haloalkane dehalogenase whose active site D106 forms an ester bond with the chloroalkane linker.
Herein, we report an alternative biochemical approach to haloalkane detection based on a set of coupled enzyme reactions.
Therefore, the majority of current haloalkane assays is focused on the monitoring of proton or halide release.
To evaluate the feasibility of this method, a set of haloalkane substrates for R. erythropolis DhaA was chosen (Koudelakova et al., [2011]).
In conclusion, we have developed a fast, simple and sensitive detection of haloalkanes and haloalkane dehalogenase activity based on coupled enzymatic reactions.
The pH indicator dye-based colorimetric method and multivariate experimental design were used for the systematic biochemical characterization of the broad-specificity enzymes haloalkane dehalogenases.
