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On the same day, CD14+ monocytes from controls were isolated and half were stimulated with 500 U/ml IFN-α2a for 48 h at 37°C in 5% CO2 with rotation to prevent adherence to plastic.
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Similarly, at OD600 nm 1.2, the untreated culture was again split and one half was stimulated with 5 mM D-glucose and grown under the same conditions as the untreated control culture for 360 min. Ten ml samples were collected 5, 30 and 360 min after stimulation.
Following, half of wells were stimulated with LPS at 10 μg/mL (final concentration) while the other half were used as control for each sample.
Within blocks of 7 s, half of the regions were stimulated with checkerboard patterns contrast reversing at 8 reversals per second, while the other half remained inactive at uniform luminance.
Half of the animals were stimulated by PEMFs (75 Hz, 1.5 mT, 4 h/day) and at 40 d, macroscopic, histological and histomorphometric analyses were performed to evaluate osteochondral defect regeneration.
DexVD3 DC and half of the DMSO DC were stimulated with LPS (100 ng/ml, Sigma-Aldrich, St . Louis MO, USA) at the time of antigen supplement.
Half a million freshly thawed PBMCs from trial volunteers were stimulated for 16 hours with Ag85A peptides or media alone.
The implants were stimulated five days per week for 900 sinusoidal half-cycles at a frequency of 1 Hz.
When Ba/F3-gp130 Ba/F3-gp130stimulated with IL-11/R-FP, a cellsntration 130-140 pM werestimulated for half-maximum proliferation.
Cells were stimulated 48 hrs post transfection.
If only one site is present on a receptor, the EC50 of a partial agonist (concentration at which half the response is stimulated) is the same as the KD value (concentration at which half of the receptors are occupied).
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