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Within each age category, mice were randomized by body weight; half were maintained on control diet and half were switched to MR diet containing 0.172% methionine (Dyets).
For etiolated studies, plants were grown in the presence or absence of 1% sucrose for seven days in the dark, after which half were maintained in the dark and the other half were illuminated with white light (70 μEin m-2s-1) for an additional three hours.
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At 3 weeks of age, half of the chickens in each Pi group were subjected to HS (Ta=41 °C, 60 min) while the other half was maintained in a thermoneutral environment (CN, Ta=25 °C).
At each passage, half of the cells were harvested and used for expression analysis, and the other half was maintained continuously in culture.
For growth at different pH, cultures were divided at mid-log phase (OD600≈0.5) and one half was exposed to Brucella broth with pH 5, while the other half was maintained at pH 7. Bacteria were harvested after 24 h followed by subsequent LPS isolation.
To assess the sensitivity of the EspP β domain to SDS denaturation, half of each sample was heated at 95°C for 5 min while the other half was maintained at room temperature.
In confirmation of this limited change in phenotype, this time point was associated with just 6 dysregulated miRNAs, half of which were maintained from day 4 suggesting that molecular modulation at day 4 may be key to the initiation of fibrodysplasia.
Half of the piglets were maintained intact and the other half was subjected to an ileo rectal anastomosis (IRA).
Cultures were maintained with half media changes every other day up to 18 days.
Deterioration of the ATTR amyloidosis, however, appears to be a less likely cause since the objective measures of the GI function (i.e. gastric emptying half-time and nutritional status) were maintained.
During the first half of the century several castles were maintained, or brought back into military use.
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