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Half were used in IF using anti-Oct4 antibodies, and the other half were analysed by RNA FISH.
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Half of the kidney containing the transplanted cells was reserved for immunofluorescence staining and histological examination, while the other half was analysed by quantitative real-time PCR (Q-PCR) analysis of gene expression.
One half was analysed by conventional histopathology, the rest snap-frozen in liquid nitrogen and stored at -80°C.
One piece was submersed in RNAlater® solution (Ambion, USA) for ribonucleic acid (RNA) isolation and the remaining half was analysed with flow cytometry.
Half seeds of the parents as well as F1 half seeds were analysed for tocopherol profile.
Contaminants collected from samples of roof products exposed at seven California sites for about one and a half years were analysed for major and trace elements and carbons to assist characterization of the chemical profile of the atmospheric particles that soil each roof sample.
Individual half seeds were analysed for tocopherol profile following the method of Goffman et al. [ 36].
For all cancellous indices at least two sections from levels separated by more than 150 μm (a reduced thickness due to the limitations of using a half core biopsy) were analysed from each biopsy to avoid replicate sampling of a single surface event.
Second, denaturating high-performance liquid chromatography was introduced to replace the single-stranded conformal polymorphism technique in our laboratory when about one-half of the cases were analysed.
The Issues Interviews and corresponding half of each patient focus group were analysed together.
For comparative purposes, all traits were analysed using the half-sib applet in QTL Express [ 44].
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