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To facilitate diffusion in organ culture, the hypothalamus was cut sagittal in half and each half was transferred into 1 ml of cooled culture medium.
To simulate acidification in the endo-lysosome of cells following intracellular uptake, NPs were first incubated in a solution at pH 7.4 for 24 h, then one-half of the NPs continued to be incubated at pH 7.4, and another half was transferred to a solution of pH 5.0.
After collection of these baseline behavioral data, half of the corals remained in the normal light:dark treatment while the other half was transferred to a constant dark treatment.
The diaphragm was bisected, and one half was transferred into a custom recording chamber in which the muscle was held in place with metal pins that passed through the surrounding tissue and were inserted into a Sylguard bed.
The other half was transferred to 50% alcohol 1% formalin and shipped at ambient temperature to CDC for processing.
Cells were grown in medium containing galactose, then the culture was split and one half was transferred to medium lacking galactose to repress Scc1 expression.
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Once he arrives there he insists the other half is transferred directly into his business account.
Then, half of them were transferred to -K nutrient solution (lacking K2SO4) as the K+-deficient treatment (LK), and another half were transferred to normal solution (+K) as control (CK).
When the transformants reached exponential growth, at OD730 near 0.5, half the cultures were kept under red light and the other half were transferred to simultaneous red- and green-light illumination, as described above.
Other plates were incubated at 28°C until the colonies were 3 4 mm in diameter at which time one half were transferred to 36.5°C and subsequent radial growth monitored for up to 18 days.
Over half were transferred from their home communities to a tertiary care facility for relatively prolonged admissions, although in most children the length of stay was 9 days or less.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com