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The resulting elution was split into two, and one half was supplemented with the PKA inhibitor PKI (10 µM).
Extracts were split in two and one half was supplemented with cytochrome c and dATP, whereas the other half remained untreated.
At OD600 nm 0.4 the culture was split, and one half was supplemented with 1 mM cAMP (LB medium contains yeast extract and will contain trace amounts of cAMP, e.g. see Polnisch and Hofmann) [ 14].
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Articular chondrocytes from rats were seeded into several flasks, and the media in one-half was supplemented with glucosamine at 20 mM.
Fertilizers were either distributed homogeneously throughout the pot ('whole') or heterogeneously, such that only one-half of the pot was supplemented with fertilizer ('split'split
The other half of the culture was supplemented with galactose.
After 2 weeks of hydroculture, the half-strength Hoagland's solution was supplemented with 200 mM NaCl.
Output in 2014 increased by over 17% and this was supplemented by nearly half a billion imported bricks, mainly from the Netherlands and Belgium".
The scientific program was supplemented with a half day excursion to Wechselburg in Saxony where fellows and lecturers learnt about the river Mulde, a previously heavily contaminated river in a now scenic, tourist-friendly setting, which is slowly recovering (Figure 4).
For half- and quarter-embryo rescues, KSOM was supplemented with 2i (1 μM PD0325901 and 3 μM Chiron; Cayman Chemical).
In nearly half of the sample, the baseline work-up was supplemented by a technetium-99m methylene diphosphonate bone scan.
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