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Blood, tumors, and several organs were halved; each piece was weighed, and half was snap frozen for quantification of the IR, while the other half was immediately prepared for gamma quantification.
One half was immediately flash frozen in liquid nitrogen for future RNA extraction.
The liver was bifurcated with a sterile surgical scalpel and one half was immediately snap-frozen in Tissue-Tek O.C.T. compound (Sakura Finetek).
The other half was immediately fixed in 10% buffered formalin for 24 h and then paraffin-embedded for morphological study using hematoxylin-eosin and PAS staining.
The left half was immediately flask frozen in liquid nitrogen and stored at −80 °C.
One half was immediately frozen in liquid nitrogen for subsequent extraction of RNA.
Similar(45)
Almost half were immediately alert upon resuscitation, while the majority, 80percentt, showed signs of brain awakening by day three, as commonly expected.
In the present study, the tumor samples were immediately divided into two parts: one-half was immediately snap-frozen in liquid nitrogen, and the other was immediately fixed.
Brains were rapidly removed, cut along the midsagittal plane, and half the brain was immediately frozen and the other half drop-fixed in 4% paraformaldehyde for 48 h.
He completed his Ph.D. in three and a half years, and was immediately hired by his department.
Dundee looked on top in the first half and it was immediately evident why manager Neil McCann placed so much trust in the relatively unknown Australian winger Jesse Curran.
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CEO of Professional Science Editing for Scientists @ prosciediting.com