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From each SLN cell sample, half was used for immediate analysis and half was analyzed after cryopreservation and thawing.
To compare these two techniques, half of each sample was analyzed using SMART-FCM and the other half was analyzed using the plate counting technique.
For each C57BL6/J cohort, half was analyzed at 3 months of age while the other half was followed until 9 months.
The remaining filter was divided in two equal parts: one half was analyzed by means of ion chromatography to determine the concentrations of particulate sulfate and nitrate; the other half was analyzed by a HPLC-fluorescence method for detection of a group of signature PAHs as previously described (Li et al. 2003).
Half of the 1.0-mL extract was derivatized with diazomethane [see Supplemental Material (doi 10.1289/ehp.1001965)] to enable the identification of haloacids (through their corresponding methyl esters); the other half was analyzed directly for other DBPs.
They bisected 162 LNs from 32 patients: the half of each section containing the largest dimension of the LN was examined with H&E staining, and the other half was analyzed by the OSNA assay and Western blot analysis.
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When 3,4-dihydroxypyruvate (product of L-dopa half transamination) was analyzed under identical conditions, the compound eluted as a sharp peak and did not co-elute with the broad peak (not shown), eliminating the possibility of the broad peak as the product of L-dopa half transamination.
Two half-sessions were recorded each day, and each half-session was analyzed separately (see Materials and methods).
mRNA half-life was analyzed as described in Leclerc et al. [53].
For study 2, the effect of the breed on cimicoxib plasma half-life was analyzed with a one-way ANOVA with the breed as fixed effect factor.
Statistical comparisons between groups were conducted by student t-test and protein half-life was analyzed using one-phase exponential decay model in Prism (Version 4.0c, GraphPad software Inc, La Jolla, CA, USA).
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