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Half of the kidney containing the transplanted cells was reserved for immunofluorescence staining and histological examination, while the other half was analysed by quantitative real-time PCR (Q-PCR) analysis of gene expression.
One half was analysed by conventional histopathology, the rest snap-frozen in liquid nitrogen and stored at -80°C.
One piece was submersed in RNAlater® solution (Ambion, USA) for ribonucleic acid (RNA) isolation and the remaining half was analysed with flow cytometry.
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Half were used in IF using anti-Oct4 antibodies, and the other half were analysed by RNA FISH.
The distal half of chromosome 1p was analysed with 15 polymorphic microsatellite markers in 683 human solid tumours at different locations.
The terminal elimination half-life of ssHHT was analysed as a function of ssHHT dose level using the Kruskal Wallis test.
One half of each skin biopsy was analysed for the presence of B. burgdorferi DNA.
The X chromosome was analysed separately because half of our hybridizations are sex-mismatched and the Affymetrix and NimbleGen CNV detection software are unable to correct for this.
The half-life of the antibody response was analysed in a repeated measurements analysis using log-linear regression model including adjusting for associations between multiple data points from the same subjects.
Protein abundance (Ghaemmaghami et al., 2003), translation rate per protein species (Arava et al., 2003) and protein half-life (Belle et al., 2006) was analysed based on data collected during non-stress conditions.
Therefore, only the upper half of beam, − h/2 ≤ z 1 ≤ 0, was analysed.
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