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Half volume of the medium was changed every 2 3 days.
Half volume of the medium was changed every 2 3 days and neurons were differentiated up to 3 months.
The tests involve three stages including the empty model digester (EMD1) subjected to accelerations of relatively small peak ground acceleration (PGA), the model digester filled with half volume of water (WMD) subjected to accelerations of medium PGA and the empty model digester with water taken out (EMD2) subjected to acceleration with PGA up to a large value.
Half volume of the test substances in each well from the upper row was transferred to the second row and mixed with the same volume of CSM medium.
Each enzyme sample (3 μL/12 μL) was adjusted to desired pH by adding a half volume of buffer with different pHs: 0.2 M citrate buffer (pH 4.0), 0.2 M phosphate buffer (pH 5.0, 6.0, 7.0, 8.0, or 9.0) or 0.2 M CAPS buffer (pH 10.0).
Each enzyme sample (1.0 μg) was adjusted to desired pH by adding a half volume of buffer with different pHs: 0.2 M citrate buffer (pH 4.0), 0.2 M phosphate buffer (pH 6.0, 7.0 or 8.0) or 0.2 M CAPS buffer (pH 10.0).
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DNA was precipitated from the aqueous phase in the presence of 0.3 M NaOAc pH 7.0 and two and a half volumes of ethanol.
The impending tax legislation has altered the mix of issues being offered, pushing the first-half volume of general obligation issues up 45.7percentt from the similar 1985 period.
After 3 days of incubation, one-half volume of fresh medium containing 2× doses of IL4 and GM-CSF was added to each well.
After adding one-half volume of fresh Luria-Bertani broth (pH 8.0) containing 1.0% L-arabinose, the incubation temperature was increased to 37°C, and the incubation was continued for an additional 4 hours.
For immunomagnetic enrichment, one-half volume of Cell Buffer (Immunicon) was added to a blood sample.
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