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Half of the cows were supplemented with a mineral/vitamin mixture (1 kg/ d of the mix in a corn/molasses carrier) and the other half supplemented with a corn-based concentrate (1 kg of concentrate per 4 kg of milk).
However, only a third of parents recalled receiving any recommendation and of these under half supplemented with vitamin D. In contrast to our study, where < 5% of caregivers thought supplementation was unnecessary, 67% of parents in the Seattle study believed that supplementation was unnecessary because breast milk has all needed nutrition.
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Articular chondrocytes from rats were seeded into several flasks, and the media in one-half was supplemented with glucosamine at 20 mM.
Extracts were split in two and one half was supplemented with cytochrome c and dATP, whereas the other half remained untreated.
"I feel like this could save my life," said Ms. Smeaton, 47, who has cut her tobacco smoking to a pack and a half daily, supplemented by her e-cigarette.
From ages six to ten, Obama attended local Indonesian-language schools: St. Francis of Assisi Catholic School for two years and Besuki Public School for one and a half years, supplemented by English-language Calvert School homeschooling by his mother.
The resulting elution was split into two, and one half was supplemented with the PKA inhibitor PKI (10 µM).
At OD600 nm 0.4 the culture was split, and one half was supplemented with 1 mM cAMP (LB medium contains yeast extract and will contain trace amounts of cAMP, e.g. see Polnisch and Hofmann) [ 14].
Transgenic and wild type seeds were pre-germinated and placed on agar plates with half MS media supplemented with 10 mM and 20 mM MG.
Medium composition for each ventilation closure treatment consisted of half strength MSBM supplemented with 0.1 mg/L−1 NAA, 3% sucrose and 0.3% gelrite.
The maximum average number of multiple shoots (9.1 shoots/explant) could be induced in shoot apices on half strength MSBM supplemented with 2.0 mgL−1 BA, 3% sucrose and 0.9% agar, followed by 8.8 shoots/explant on medium with 1.0 mgL−1 BA.
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