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The cells were cultured at 37°C and 5% CO2, and half of the medium was changed every 72 h.
Half of the medium was exchanged every second day.
At day three, half of the medium was replaced.
Half of the medium with cytokines was replaced every 2 days.
On day 5 of the expansion, half of the medium was replaced by fresh medium.
Half of the medium was replaced on day three and six.
Half of the medium was exchanged every 3 days with fresh medium.
Half of the medium was replaced once a week with fresh medium.
In the second week, half of the medium was exchanged with fresh one.
At day 6 of coculture, half of the medium was replaced with fresh medium supplemented with cytokine.
Every 3 4 days, half of the medium was discarded and replenished by fresh medium and cytokines.
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CEO of Professional Science Editing for Scientists @ prosciediting.com