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The other half of cells was fixed in 70% ethanol for at least 1 h.
In the lowest concentration of ZnO NPs (175 μg/ml), more than half of cells remain survive (55.5%).
Two days later, half of cells were harvested and followed by protein extraction and Western blot analysis of PRL-3 expression and the other half of cells were treated with different dose of ABT-869.
Samples containing half of cells in each well were run on SDS-PAGE or native agarose gels, and analyzed by Western blot using anti-Dd antibody.
After removal of RO-3306, cell number increased by 53% (3 replicates, 1151 cells at G2/M, 1757 cells at 3 h) in the next 3 hours, indicating around half of cells accumulated at G2/M during overnight synchronisation.
Moreover, almost half of cells survived even after exposure for 48 hours.
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Half of cell phone owners use mobile banking once a week.
More than half of cell phone internet users (55%) go online from their mobile phone on a daily basis.
Additionally, at day 4 almost a half of cell population was dead as estimated with methylene blue staining (Fig. 4).
About half of cell line samples were predicted to have some degree of aneuploidy, including 24% and 62% of cell lines derived from normal and cancer tissue, respectively.
At day 4, almost a half of cell population was apoptotic and at day 7 we were unable to record apoptosis (Fig. 5).
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