Cell suspension was transferred to sterile glass Petri dishes, each of which contained one piece of sterile glass slip (half of a microscopic glass slide; 38 mm × 26 mm), and incubated at 18 °C for 48 h to allow the bacteria to attach to the dish surface.
The DNA sequences from Figure 6 were plotted using the same PWM model counting the number of half sites over a sliding widow size of 250bps.
Slides were recoded, and another 5,000 sperm were scored from the bottom half of the slide, for a total of approximately 10,000 sperm per slide.
According to the experimental protocol of the ICPR 2012 contest, the ICPR 2012 dataset is divided into a training set with 721 cells from half of the slide images and a test set with 734 cells from rest of the slide images.
Kidneys from wild-type, heterozygous, and homozygous Farber mice were sectioned in duplicate, with half of the slide sprayed with bCDase at a concentration of 8 mU and the other half covered by a glass slide as a control.
An index of health care shares accounted for half of the slide.
We further categorised which locations had high seasonal variation in transmission, defined as the slide-prevalence in the low season being less than half of the slide prevalence in the high season (n = 10 locations).
Then, one drop (5 μl each) of a urediniospore suspension (86,000 spores ml-1) of a H. vastatrix isolate compatible with the BI625 genotype of C. arabica was added to each half of the slide after being previously subjected to ultrasonication for 30 s at 60 MHz to allow for dissociation of the spores.
Five thousand sperm were then scored from the top half of the slide.
Cut sections and place on clean, room temp, plain glass slides (orient sections in the middle toward the lower half of the slide).
Targets were evaporated in a Speedvac, resuspended in hybridization solution (50% formamide, 25% Amersham Microarray Hybridization Buffer V.2, 25% H2O) and manually hybridized for 16 h at 42°C to microarray slides containing 4,608 different cDNA probes, each spotted in duplicate on either half of the slide.
