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The plants were in a four and a half leaf stage and almost of same size.
We discussed using some Chinese combination herbal agents, but eventually we decided to simply add one half leaf of organic kale to the diet by blending it to a puree with a bit of chicken broth and then mixing this with the dog's regular diet.
Each harvested leaf was bisected and one half used for the nuclear area measurement, while the remaining half leaf was processed for flow cytometry measurements (see below).
Epidermal cell size and number of cells on the adaxial side were determined in the middle region of a half leaf near the midvein.
It is therefore possible that ROS generated in the half leaf that transiently expressed the SlMKK2 DD or SlMKK4 DD construct may trigger the generation of yet unknown systemic signal(s), which transduce and activate defense responses in the opposite half leaf.
Biotic stresses included; bacterial (LPS, HrpZ, Flg22) and oomycete- NPP1) derived elicitoomycete- NPP1cinerea infection, Erysiphe orontii infection, Phytophthora infestans treatederivedonselicitorslent, avirulent, type III-secretion system deficient and non-host Botrytis and half leaf Pseudomonas treatment.
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The anti-TMV activity was tested by half-leaf method.
DDN, a lycoricidine alkaloid, showed strong activity against TMV by the half-leaf method, the IC50 was 1.80 μM [5].
Some of the compounds and lycoricidine derivatives had been screened for the activities against tobacco mosaic virus (TMV) by the conventional half-leaf method.
The inhibitory activities of compounds 1, 2, 6, 8, 10 13 and lycoricidine derivatives (L1 L7) (Fig. 2) against TMV were tested by the half-leaf method and reported in Table 1, all of the compounds exhibited moderate inactivation effect.
Using growing N. tobacum L. leaves at the same age as the test subjects, the curative and protective activities against TMV were evaluated based on the half-leaf blight spot method [25 27], and the commercial agent ningnanmycin was tested as the control under the same conditions.
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