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PCR amplicons of these regions were used to prepare the tandem clones with site directed mutagenesis used to make a perfect in frame fusion of the duplicated half junction.
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The crossroad causes a high drop of wireless signal, but not enough to completely prevent reception and, in the last case, half of the junction is still covered (the last reception takes place at about 60 meters from the center of the crossroad).
Half of the junction's ramps directly connect to CR 13 while the others use one of the parkway's two service roads, named Spur Drive South and Spur Drive North.
Craig Rance, a spokesman for Thames Water, said: "A 3ft-wide water pipe burst in Herne Hill at the junction of Half Moon Lane at around 5am today.
The company said the 3ft-wide (0.9m) burst pipe had been located at the junction of Half Moon Lane.
One Hobo U12-006 by Onset USA was equipped with a TMC20-HD sensor and was used to monitor the temperature of the spring water; it was inserted for about half meter in the junction of floor blocks to reach the water.
The end of an exon was defined as 100 bases from the intron/exon junction, or half of the exon if it was less than 200 bases in length.
To validate the mode of a PA cluster, a junction primer (+, half complementary to 3′ end sequence and half complementary to poly(A) sequence) together with an upstream gene-specific primer were designed by Primer3 (version 0.4.0) and synthesized by IDT.
In a randomized-contact decoder (RCD), for example, field-effect transistors are randomly created at about half of the NW/MW junctions.
He sent the tank company ahead first, which initially reported no Egyptian units, and Shunari dispatched an infantry unit in six half-tracks to the junction.
Half an hour past the junction, I started to wonder if I'd taken the right turn.
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