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Further screening with one and half fold change cut (FC ≥ 1.5) showed 82 and 62 differentially expressed miRNAs in 8 mg/L and 20 mg/L test concentrations of NaF, respectively.
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Spots with one and half-fold change or more were considered differentially expressed using p value < 0.05.
Of the 70 most differentially-regulated transcripts (fold change < -4), half had a significant match against a known protein/DNA sequence.
Approximately half of the genes exhibited fold changes > 2, and half exhibited less than a 2-fold change.
Fold change.
FC, fold change.
Log2 fold change.
Fold change (qRT-PCR) Fold change log2 FC) FDR Sig.
Legend: LogFC, Log Fold Change; MA, microarray.
Numbers in parenthesis indicate the fold change.
Fold change was calculated [23].
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