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Similarly with heads dissected in half and bodies dissected in half, expression was observed in all tissues, including abundant expression in nervous system, muscle (including flight muscle), and fat-body tissue.
ST3Gal.II transcript levels were higher in the MCR and T24 cell lines (6.1 ± 0.4 and 7.5 ± 0.4, respectively), followed by the 5637 cell line, which showed around half expression, and the HT1376 cell line which showed one tenth of this expression (Fig 3A).
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Just after seven o'clock, Peter Gelb, the general manager, walked before the curtain, his face betraying a half-rueful, half-gleeful expression, to announce that this would be not only Lehman's Met début but also his first attempt at the role.
In this system, the behavior near half maximal expression is biologically most relevant, since it is at this very concentration that the system has to 'decide' where to draw a fairly sharp gene expression boundary as one of the first steps in constructing a spatial pattern.
The half maximal expression point occurred at ∼12 DIV.
Again, 1/ x of a worker's sisters will be full sisters, and the remainder half sisters (expression 3).
Bacterial cultures were then divided into halves: in one half, protein expression was induced with 0.2% arabinose, while the other half was left untreated.
We demonstrate that P. interpunctella lacks complete Z chromosome dosage compensation, female Z-linked genes having just over half the expression level of males and autosomal genes.
The single copy of the Z chromosome possessed by females equates to on average slightly more than half the expression level of males, as observed for the majority of female Z-linked contigs in Plodia.
Graphical inspection indicated a nonlinear relationship, with a positive correlation only given for approximately the lower half of expression levels above which (at FPKM ≈ 24 = 16) the correlation seemed to level off (fig. 1; supplementary fig. S6, Supplementary Material online).
Our analysis shows that P. interpunctella lacks complete Z chromosome dosage compensation, showing approximately half the expression in females compared with males for Z-linked contigs, the largest dose effect related to sex chromosomes yet observed.
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