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Although we cannot exclude the possibility that other molecular processes are involved in the unc-51 mutant phenotypes, the weaker phenotypes of the double heterozygotes might be accounted by partial suppression of the gene activity given that wild-type alleles are retained at a half dosage for both genes.
Similar results, corroborating the presence of putative deleted genes at half dosage, were also obtained for the 39E7 mutant.
We used half the dosage reported in the literature to test the efficacy of doxycycline even at half dosage to lessen collateral effects [ 14].
Real-time PCR quantification of gene dosage for some of the BAC ends confirmed the presence of these sequences at half dosage in the mutant genotype, indicating that the 39B3 mutation is a hemizygous deletion.
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To determine synergistic effects of MMR-A7 and MMR-B3 knockdown, a transfection was performed in which MMR-A7 and MMR-B3 at half dosages were together complexed with the lipofection reagent (Snell TW, Shearer TL, Smith HA: Exposure to dsRNA produces RNA interference in Brachionus manjavacas (Rotifera).
However, half-dosage was significantly less effective than the full dose treatment (Supplementary Fig. 7).
They sometimes share their pills, cut them in half and take half-dosages.
Foxp2δ 12 14 /δ 12 14 homozygotes lacked the FOX domain-encoding exons – primer pair 13/14 yielded no product in these embryos, and a half-dosage in Foxp2δ 12 14 /+ heterozygotes (Fig. 3a).
In case of adverse effects which seem attributable to mefloquine, students are advised to use half the dosage twice weekly instead of the standard full dosage once a week in order to lower the peak plasma concentration [ 15].
"The standard rule of thumb," he said, "is to give Asians half the dosage".
"Seven of Wal-Mart's top 10 states have a higher unemployment rate than the national average," Mr. Quinn said, including California, Florida and Ohio, and shoppers are talking about "adding water to their milk and taking half the dosage of their prescriptions".
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