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The term apparent dissociation constant is used in place of the half concentration of excitation and inhibition.
After a 2 min incubation in this buffer, the actin solution was combined with an equal volume of Arp2/3 complex and VCA materials in double strength KMEI buffer (such that the final solution was 10 mM imidazole pH 7.0, 50 mM KCl, 1 mM EGTA, 1 mM MgCl2, 0.5 mM DTT, with one half concentration of buffer G carrying over from the actin solution).
Of note, 84.6% of embryos injected with half concentration of exon 9 morpholino exhibited the same phenotypic (both motor and morphologic) changes (n = 78, data not shown).
Seeds used in all the assays done on seedlings were sterilized as previously described [ 73], placed on either on Murashige & Skoog (MS) media (Research Products International Corporation, Mt. Prospect, IL) with 1% plant agar in Petri plates or on half GM plates (half concentration of MS salts, 1% sucrose, 0.8% Plant agar, pH 5.7).
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The rate equation for the transesterification reaction in the model system was modified using half-concentration of BEN, which is the only effective in the intermolecular exchange.
In another set of experiment cells were incubated with half concentrations of compounds and predinsalone was used as reference drug (control positive) with concentration of 10 μg/mL.
At GD20 the prenatal brain contained half the concentration of DA in the amniotic fluid, and was approximately half that found in the brain of the dams.
Subsequent expansion was performed in the same medium with half the concentration of these cytokines.
Stems contained slightly more proline than leaves, with roots containing about half the concentration of leaves.
Usually, BW25113 produces about half the concentration of DH1 (Niwen Kong, personal communication).
Subsequent expansion was performed in StemSpan medium containing half the concentration of the above-mentioned cytokines (TSF6half medium).
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