The bar-code strands with the half-complementary biotinylated DNA and half-complementary DNA-modified gold nanoparticles probe were immobilized onto the surface of a streptavidin-coated micro well plate, and the signal was amplified by silver enhancement.
The small dashed-lines denote un-fixed conformation, where the base position belonging to this region can be either paired or unpaired as long as other mandatory regions (i.e., the base pairing of CUGAUGAG-region and the complementary half of the helix II, effector 1 (E1) with its binding site and effector 2 (E2) with its binding site) are present.
YN-LeEix2_CD and all forms of AtEHD2 were examined for fluorescence with the complementary half of the YFP protein and the results were negative (Figure 4 and [6]).
We also verified the known interaction between the two subunits of AP-2, μ and o', in the BiFc system – see Figure 9. YN-LeEix2_CD, YC-AtEHD2 and all Adaptin proteins were examined for fluorescence with the complementary half of the YFP protein and the results were negative (Figures 4,8).
Two strains of mice were generated with complementary half deletions of the conserved WBS orthologous region in mouse and crossed to generate mice carrying both deletions.
A heptanucleotide, corresponding to each half of a complementary sequence of a 14-nucleotide target sequence (T0 in Fig. 7C), was appended onto the 5′ terminus of one domain and the 3′ terminus of the other domain as a target-binding arm (Fig. 7C; I-bin and II-bin).
Moreover, the proposed structure possesses a favorable 2-D doubly complementary half-band (DC-HB) property that allows about half of the 2-D NSHP recursive DAF's coefficients to be zero.
The structure also possesses a preferable doubly complementary half-band (DC-HB) property that reduces the number of required independent coefficients for designing 2-D interpolation/decimation filters.
We generated two strains of mice with complementary half-deletions of the conserved Wbscr on mouse chromosome 5G2 by using chromosome-engineering techniques in mouse embryonic stem cells (ESCs) (Fig 1B).
Each probe sequence was complementary to half of the sequence of a third strand, the "target" strand that the sensor is designed to detect.
