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The carcass is sawed in half and washed with a lactic-acid solution before it's moved to a chilling compartment.
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The standard testing strategy, also applied to the grebe, was to cut the swab in two, freeze and store one half, and wash the other in 500 μl TE (ph:8) to recover any virus present.
Four coated plates were kept at room temperature for half an hour and washed five times with PBS-T (phosphate-buffered saline); 50 μL of diluted patient serum samples and positive and negative controls (1 100) were added into respective wells and incubated at 4°C overnight in a moisture box.
Wait twenty minutes to a half hour and wash your hair normally.
The supernatant was mixed with a half volume of ethanol, loaded and washed through the RNeasy mini kit column (Qiagen).
After 30 min, the reaction mixture was diluted with methylene chloride and washed with half-saturated sodium bicarbonate solution.
The leaf discs and roots were stained with 5 μM MitoSox-Red (Invitrogen) for 25 min and washed with half-strength MS medium before viewing.
He spends five and a half years shoveling snow and washing dishes and tending to the needs of everyone around him.
Place (in most jurisdictions) "inch-and-a-half washed drain rock" from a nearby gravel pit around the pipe.
After separating capsules and fats, the right kidneys were halved, one half was washed in cold PBS and stored at -20°C until vitamin E measurements were obtained, and the other half was fixed in 10% formalin for histological evaluations.
After the incubation time, half of the culture was placed in a 96-well tissue culture plate (modified 48-h assay), and the other half was washed three times with 1,000 μl of RPMI medium to remove DHA, before being replenished with drug-free medium and placed in a 96-well culture plate.
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CEO of Professional Science Editing for Scientists @ prosciediting.com