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Because it is difficult to detect any differences in virulence between groups due to the rapid death of the infected larvae at that dose, we reduced the dose by half and inoculated the larvae with 5×105 dead and 5×105 live conidia per insect.
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Each half was homogenized and inoculated into mice.
To study sideward movement of the resistance-activating substances, mycelium extract was applied to the left or right half of pepper leaves and inoculated with zoospores of P. capsici 0, 5, 10, 15 mm away from the treated area (Figure 1).
Each well was half-filled with PDA and inoculated with T. virens.
Within each treatment, we fed half the adult females on a mix of P. berghei ookinetes and blood and inoculated the other half with a negatively charged CM-25 Sephadex bead to evaluate the mosquitoes' melanisation response.
After 5 weeks of As exposure, mice were anesthetized with 9 1 ketamine:xylazine mix at 0.1 mL/30 g body weight and inoculated intranasally with one-half the median lethal dose of standard laboratory influenza A virus A/PuertoRico/8/34 (H1N1) strain (Flu).
The half-life (t1/2) of atrazine decreased from 14 to 6 days for the control and inoculated samples respectively.
The queens are assessed, and if necessary, replaced with stronger ones, and the bees are fed and inoculated against disease.
T SH prepared and inoculated Arabidopsis plants.
The right half of each leaf portion was inoculated with 3 agar plugs containing actively growing Phytophthora capsici mycelium and the left half was inoculated with sterile agar plugs as negative control.
One half was inoculated into BSK-II media to obtain isolates.
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