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Taken together, our analysis of GFP and GST fusion proteins shows that EF-G1 has two localization signals, an N-terminal mitochondrial target signal and a C-terminal nuclear signal.
Less amphiregulin staining was seen in the nucleus, even though having two nuclear localization sequences (NLS) within the DNA sequence [12].
Menin has three nuclear localization signals (NLS) near the C terminus of the protein and the novel M561K mutation identified lies within the second NLS domain.
LKB1 also has two nuclear localization signals (NLSs) in its N-terminal region, and LKB1 without an NLS remains in the cytoplasm, even though wild-type LKB1 is localized to both the cytoplasm and the nucleus (Sapkota et al. 2002).
The response screen for the menu localization task had two green buttons, labeled "side" and "top," beneath the text, "Was the menu on the left side or the top?" Performance was high on this task (M = 84.6%, SD = 7.1), and each subject was significantly above chance (p < 1E-5 per subject, chance = 50%).
Testing why VpCDPK9 has four patterns of localization, and the relationship between its localization and function, will require more experimental data.
The Arabidopsis U1-70K has two independent nuclear localization signals (NLS) located in the N-terminal and C-terminal half of the protein.
Forkhead box (FOX) P2 involved in a speech/language disorder has two separate nuclear localization signals.
PPM1D605 has two putative nuclear localization signal (NLS) domains and is assumed to be a strictly nuclear phosphatase, while PPM1D430 contains only one NLS and shows both nuclear and cytoplasmic localizations.
Nuclear medicine imaging of infection has two major indications: localization of a focus of infection in patients with fever of unknown origin; and the diagnosis of an infection in patients with localized symptoms, for example after surgery, when normal anatomy is absent or when metal implants prevent computed tomography or magnetic resonance imaging.
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