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Our review of all known IDE variants identified 15 additional variants, each located in a 100 bp region that had sequence identity of ≥70% between the mouse and human sequence.
Reads with hits were assigned to the genome corresponding to their top BLAST hit only if the top hit had sequence identity >95% and the next highest hit to a different organism had a bit score at least 20 points lower.
Many structure based alignment softwares were employed to create reliable alignments between distantly related proteins, as no two sequences in a superfamily of PASS2 had sequence identity of more than 40%.
To avoid the redundancy of the benchmarks, the proteins were selected so that this set and the previously used 36 target proteins had sequence identity between all the pairs of < 30%.
Genomic analysis showed that these isolates had sequence identity of >99% in each of the 8 genes tested with human isolate A/Jiangxi-Donghu/346/2013 (H10N8) (JX346), which was derived from the case-patient who became ill in December 2013.
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Of thirty-two OTXer OTX homeodomain sequences, Ap-OTX2 homeodomain had sequence identities ranging from 72 98%.
These sequences occupy different locations within the phylogenetic tree and had sequence identities of only 22-24% to MptpB.
The cluster B had 16 miRs each with a 13-nucleotide length which had sequence identities with viruses.
Of these genes, 16 had sequence identities of greater than 95%, 137 genes with identities between 90% and 95%, 120 genes with identities of 85%to90%0%.
Hits were counted which had sequence identities greater than or equal to 70%, 80 %, 95, 97%, 97%, or 98% corpared to the query.
Two TDFs have sequence identity to genes known to have function in plant defense.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com