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For the complementation of the atnup62 mutant, AtNUP62 cDNA was amplified by PCR using oligonucleotides 5′-TTGTAGGTCACCTCAAGACATCCAGTGCTTTGGAGCC-3′ and 5′-ACCCGCCATGGCGGGGTTTCCATTTGGTCAATCC-3′, digested with NcoI and BstEII, and introduced into pCambia vector that had previously incorporated 1.8 kb upstream region from AtNUP62 gene with NcoI site at its 3′ end.
And it strikes me that one reason why politics in the US have become so much more bitter over the past couple of decades is that two rather different threads of religiosity have come to dominate the two major parties in distinct fashion, where each party had previously incorporated major components of both.
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The court has previously incorporated this perspective in its opinions on the death penalty and life without parole for juvenile offenders.
For his "Hobbit" movies Mr. Jackson has previously incorporated Tolkien characters who didn't appear in the novel, like the elf Galadriel (who will be played by Cate Blanchett, reprising the role from the "Lord of the Rings" movies).
We have previously incorporated the rtTA2S-S2 gencodingding a second-generation rtTA variant [ 6] and the tetO elements into the HIV-1 genome to control virus replication [ 21, 22].
The music of the national anthem, created by Alexander Alexandrov, had previously been incorporated in several hymns and compositions.
A subset of the markers that were identified as polymorphic in the Steptoe vs. Morex comparisons had previously been incorporated into a linkage map for this population [ 20].
The relative contrast in hybridization intensity of a subset of markers that had previously been incorporated into a DArT consensus map for barley [ 20] were plotted against the markers' positions in the barley genome.
Fluxes were dominated by dissolved silica (900 13,800 μmol m−2 d−1), ammonium (430 3360 μmol m−2 d−1) and ortho-phosphorus (50 1200 μmol m−2 d−1) and were greater than literature values which had been previously incorporated in local ecological modeling efforts.
Nanodiamonds have previously been incorporated into polymer matrices to improve mechanical properties and/or elute therapeutic compounds.
The MTA vincristine has also demonstrated therapeutic efficacy in myeloma, and has previously been incorporated into initial treatment regimes for newly diagnosed patients (Alexanian et al, 1990).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com