Subsequently, null alleles of Spt5 were characterized in zebrafish that had originally been generated in a large-scale screen for mutations affecting embryogenesis 32, 33.
Additional sequences were obtained from Genbank and had originally been generated by Geiger & Ranker [ 18], Li & Lu [ 19, 20], Ebihara et al. [ 64], de Groot et al. [ 70], Juslén et al. [ 57], and Sessa et al. [ 27, 58].
Mice deficient for BAX or BAK had originally been generated on a mixed C57BL/6 × 129SV background, using 129SV-derived ES cells, but were subsequently backcrossed with C57BL/6 mice for more than 12 generations.
A targeted knockout of Tgif1 was used to examine potential auditory phenotypes associated with the gene, including OM. Tgif mutant mice had originally been generated and maintained on a mixed background and were reported to be viable, fertile and with no obvious phenotype in the major organ systems (28).
Breeder SOD1G93A rats, which had been originally generated by Howland et al. [5], were obtained from Taconic (http://www.taconic.com/), and colonies were developed by crossing male founders with female Taconic Sprague Dawley rats (http://www.taconic.com/).
The entire coalescent network appears to have been originally generated by nursery genotypes, thus suggesting a significant number of introductions is likely to have been caused by the movement of infected ornamental plants both at short and long distances.
The research team almost perfectly reproduced a key-shaped object after they recorded the sounds a 3-D printer had made originally generating the object.
And at the end of it, for government, it will generate nowhere near the financial return it had originally expected".
And at the end of it, for government, it will generate nowhere near the financial return it had originally expected," he said.
The city had originally projected that the securities industry would generate $7.5 billion in profits this fiscal year, but it now expects that the total will reach $18 billion.
