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Control conditions were established by the use of autologous PBMC, which had not been stimulated with peptide, but otherwise had been treated identically.
The basal chemiluminescence of blood that had not been stimulated by PMA was also measured; this acts as a baseline or control with which to compare the individual's LCC.
As a negative control, we utilized MOCK samples which had not been stimulated with EGF.
Briefly, female frogs that had not been stimulated to lay eggs in the past six weeks were used for oocyte collection.
The S177E mutant became phosphorylated at Ser, even in MEFs that had not been stimulated with IL-1 or TNF, whereas the S177A mutant or wild-type IKKβ did not.
The mutation of Ser of IKKβ to glutamic acid (to mimic the effect of phosphorylation by introducing a negative charge) permitted the IKKβ catalytic subunit to autophosphorylate at Ser and this induced activation even in cells that had not been stimulated with IL-1 or TNF.
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Mayor Chuck Reed of San Jose has not been stimulated much by the federal economic recovery package — and neither has his city.
Panels E-H in Figure 7 are embryos that have not been stimulated.
For a synapse that has not been stimulated recently this simplifies to w = Pq.
More specifically, in synesthetics, the stimulation of one sense organ leads to an additional perceptual experience in another sensory modality that has not been stimulated at that moment.
In addition, some minor aspects were identified for future improvements, such as the pre-emergent seedling mortality in very compacted soil structures or germination rates of seeds that had not been previously stimulated by light.
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