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These mutants had no expression of sigmar but did express the l(2 dtl gene; this strain is referred to as sigmar S. To rescue the sigmar gene (ie. to create a control strain for loss of function studies) in the sigmar S strain, it was crossed to pUAST-sigmar /CyO-TM6B flies and the progeny named as sigmar res (sigmar Df-C23/sigmar Df-C23; pUAST-l(2 dtl /pUAST-sigmar (non-Tubby).
Quantitative real-time PCR (qRT-PCR) analysis of transcript levels showed that 15 of the 23 genes had no expression specificity; 7 of them were specifically expressed in some of the tissues; and one of them was not expressed in any of the tissues or organs studied.
In addition, hESC-derived cells expressing high levels of SMC markers had no expression of PECAM-1 (endothelial cell marker) and α-actinin (a marker of cardiomyocytes) (data not shown) confirming again their SMC differentiation.
Those genes which were expressed in the BM of patients but had no expression in the BM from healthy volunteers were considered to be derived from cancer cells lodged in the BM.
"In the beginning, we had no expression on our faces because we were petrified," Kokal says.
In the third instar wing disc the Ato-3.6BB-GFP had no expression (R).
Similar(24)
"He had the same brown jacket on, he was childlike in a way, he just had no expressions, nothing," she told ABC reporter Mark Reddie.
Their eyes have no expression.
They have no expression because they have no feeling.
The fact that he can't talk and has no expression seems a minor problem.
They have no expression on their faces, and within seconds they turn back to their own concerns.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com