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We determined the number of bases in each read that aligned with the corresponding contig from the top hit that had no alignment gaps; where multiple hits with equal scores existed, the first hit listed was used.
Additionally, some unigenes that had no alignment hit in the above databases were further estimated by using ESTScan (3.0.3) software (Iseli et al. 1999) to predict their protein coding sequences (CDS).
If a unigene had no alignment hit (match) in the above databases, ESTScan software [ 16] was used to predict the CDS domain and its direction.
Although misalignments due to indels may be more likely in the vicinity of LCRs, this does not appear to be a factor, because the relationship between substitution and distance to LCR was only slightly weaker (−0.7432, P value ) when only flanking regions that had no alignment gaps were used.
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8 families had no alignments between 40 and 400 nt and were hence discarded from the test set.
Of the remaining 72 RJ targets, 20 amplified either only the AL8/78 DNA target (15 out of 20) or had no alignments in the SNP locations (5 out of 20) reflecting diversity between AL8/78 and AS75 in repeated sequences.
Out of 95 sequences, 18 sequences did not amplify in both accessions (AL8/78 and AS75) and 34 sequences amplified only in one of two accessions or their target sequences had no alignments in the SNP locations.
To this end, we report an overview of recent efforts in LCD technologies devoted to realize more display modes having no alignment layer, faster switching time and low battery consumption.
Echoing subprime, the college bubble even has its own version of the "principal-agent problem," in which the financial interests of the college (which profits from the loan money) have no alignment with the students (who voluntarily drown themselves in debt).
If the answer has no alignment or mention of how they worked with a team to accomplish the goal, then you've identified your first great employee.
Additional protein-coding gene models from other teleost genomes (medaka and zebrafish) were mapped onto cod genome regions having no alignment with stickleback (Supplementary Note 17 of [ 3]).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com