Among the 234 (94.4%) successfully amplified markers 80% had median coverage within one order of magnitude, indicating relatively uniform performance; coverage uniformity across individuals was also high.
Since operon identification was performed only for genes having median coverage higher than two, variations in number of transcriptional units (TUs) among different growth conditions could be influenced by the number of sequences obtained for each sample.
The top 100 recurrently mutated loci in TCGA lung adenocarcinomas (Fig. 3a) had a median coverage of 3% (i.e. mutated in 3% of TCGA patients).
We reconstructed the genomes of 110 individuals from the wild-type population and of 106 from the recq4a population; these had average and median coverages of 0.6x and of 0.4x.
Despite the climate, Racine had median listing price appreciation of 16% over the last year with its median DOM down 13%.
The coverage statistics for the resulting dataset have a mean of 34.5 and a median coverage of 23 at each CpG site.
The male and female sequencing libraries had a combined 1,289,142,362 nonduplicate reads, with a median coverage of 52× and 47×, respectively.
Therefore, as expected, the HGAP2 assembly had the lowest maximum coverage value (~1,100×, or 22× the median coverage) of all assemblies while the assemblies that relied on only MiSeq data have the highest maximum coverage (~15,000×, or 300× the median coverage).
Initial de novo assembly of the short insert reads was conducted in Velvet v. 1.19 [ 67] with over 156 million reads where the assembly had a median coverage depth of 74.45.
Rosetteless was sequenced to a median coverage of 71× and over 93% of the reference genome had at least 10× coverage, while the parental strain and C2E5 were each sequenced to a median coverage of 50 60× and over 91% of the genome had at least 10× coverage.
