Sentence examples for had est from inspiring English sources
21 potential STEPs had EST evidence of intron retention or exon extension, as well as population frequency data for comparison.
All the selected genes had EST expression evidence and covered six phylogenetic groups.
In silico analysis indicated that all 70 RGHs had EST support for gene expression.
Furthermore, we found that ∼6.7% of intact silkworm Helitrons (1317 of 19 580) had EST evidence.
At an E-value cutoff of e-20, 18 contigs had EST hits (Table 3).
A recent study suggested that ∼9% of maize Helitrons had EST evidence and could generate abundant transcripts through alternative splicing.
Predicted Poly(A) sites were then defined as conserved if they were within a distance of 30 bp of a properly aligned poly(A) signal and had EST-support in both human and mouse.
No individual cultivar had ESTs spanning a complete coding region.
Only 31 sequences were common among all four classes; 39 contigs had ESTs that were expressed in the two control classes (RC and SC) and 22 had ESTs common between the two infected classes (RI and SI).
Interestingly, the five contigs that had the highest numbers of ESTs (ID: 198, 15591, 1258, 1567, and 3721) also had ESTs in all five libraries.
We found 131 transcripts with ESTs mapping to their 5'-ends (Additional file 5) and 956 transcripts had ESTs mapped to their 3'-ends.
