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These microenvironments had cytotoxic effects.
Greater concentrations of control sense oligonucleotides had cytotoxic effects.
As shown in Figure 3, none of the three concentrations of the four chemicals had cytotoxic effects on HAC.
The MTT assay was used to clarify whether histamine (0.1 and 1 mM) and/or TNF-α (10 and 50 ng/ml) had cytotoxic effects on SiHa, ME-180 cells and keratinocytes.
Previous papers reported that H2O2 had cytotoxic effects in various in vitro model by a mechanism involving pro-apoptotic factors (Bax, caspases, PARP) activation and intracellular signaling pathway [ 45– 51].
Vincristine, a chemotherapeutic drug used for some cancer types, had cytotoxic effects on MDCK, A549 and HeLa with values of 145.83 μg/ml, 0.6 μg/ml and 0.39 μg/ml, respectively.
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In vitro cytotoxicity experiments proved that free SN38 and SN38-HSA conjugates had cytotoxic effect on HT-29 human colon cancer cell line.
In conclusion, the present study has shown that SN38-PEG-PLGA-Ver nanoparticles had cytotoxic effect on HT29 cells in a dose- and time-dependent manner.
According to the MTT assay and DAPI staining results, SN38-PEG-PLGA-Ver nanoparticles had cytotoxic effect on HT29 human colon cancer cells by inducing apoptosis.
Truncated HlyA peptide still has cytotoxic effects, but the molecular lesion lessens the severity of its cytotoxicity [10].
Even commercially available silver-based dressings have been proved to have cytotoxic effects on various experimental models[83].
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