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Then, the nucleotides were replaced on the detected variant position in the reference genome with the alternative nucleotides if the position (1) had coverage of least 20% of the average coverage by reads in each library, (2) covered by at most twice of the average coverage, and (3) had 30 or higher mutation detection quality (QUAL) reported by GATK.
However, this tract only had coverage of 10X by colony PCR and Sanger sequencing.
Overall, 98% of our amplicons had coverage of >150 reads.
Only 17% of these had coverage of >80%.
We used only sites that had coverage of more than 1 and less than 31 reads.
For both species, ~65% of the assemblies had coverage of >80%, indicating good homology.
Similar(34)
Human treatment coverage was high, ranging generally from 90 100% except for Jizhong and Xindong, which had coverages of 89.7% and 87.6%, respectively, following baseline (Figure 1).
How has coverage of different topics changed over the years?
6.04pm BST I have coverage of Atletico's match now.
Jump in! We'll have coverage of the event here on Scope tomorrow and Monday.
We'll have coverage of the that other semifinal soon, but for now, thanks for reading.
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