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Three of the patients with total graft failure were secondarily augmented with retromolar grafts, harvested from the contra-lateral site, and all initially planned dental implants had been successfully inserted.
Colonies showing green fluorescence were picked and PCR amplification of the GFP gene followed by gel electrophoresis showed that the GFP fragment had been successfully inserted into the expression vector.
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The backflow of venous blood is confirmed after puncture to ensure that the needle has been successfully inserted into the vein.
The amplification with these two primers is only possible if the promoter from TPI1 has been successfully inserted upstream of the ORF.
The insertion of the cassette in the right locus was checked by PCR (Additional files 1: Tables S5 and S6), where a PCR product is only obtained if the cassette has been successfully inserted.
Tirpitz had been successfully neutralised.
As shown in Fig. 2b, the vgb gene has been successfully cloned and inserted into the pBSPPcGm with the corresponding restriction enzyme sites, obtaining the pBSPPcGm- vgb.
Efficient exfoliation of well-crystallized (Y0.96Tb x Eu0.04-x )2(OH)5NO3 · nH2O (0 ≤ x ≤ 0.04) layered rare-earth hydroxide (LRH) crystals into tens of micron-sized unilamellar nanosheets has been successfully achieved by inserting water insoluble oleate anions (C17H33COO−) into the interlayer of the LRH via hydrothermal anion exchange at 120°C, followed by delaminating in toluene.
Stable BRCA1 transfectants HCC-1937 have been successfully generated by the use of wild-type gene inserted into different vectors (Ruffner et al, 2001; Andrews et al, 2002).
The lines have been successfully blurred.
Other classics have been successfully rewritten.
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CEO of Professional Science Editing for Scientists @ prosciediting.com