Sentence examples for had been restriction from inspiring English sources
Plasmid pCM1-imp was created by cloning a 1081 base pair PCR product containing the imp gene and promoter region into pCM1 that had been restriction digested with HindIII and EcoRI.
Plasmid pCM1-graRS was created by cloning a 1751 base pair PCR product containing the graRS genes without their native promoter into pCM1 that had been restriction digested with KpnI and EcoRI.
Jahan Wilcox, a spokesman for the E.P.A., said that there had been restrictions on the number of news media representatives that could attend the event because of the size of the room.
The most used M. avium typing method has been restriction fragment length polymorphism (RFLP) [ 18].
A total of 35,000 clones from these libraries (average insert 182 kb, ~5.6× genome coverage), have been restriction fingerprinted and assembled into 3,600 contigs [ 20].
A spokesman for Stansted said: "As a result of this incident and low visibility there have been restrictions on arriving flights.
POMPEO: I'd prefer to defer the details of that to the classified setting, but there have been restrictions on coal that have been significant.
The respondents argue, however, that in so far as there have been restrictions on the right to be heard and the right to effective judicial review, these restrictions are justified.
However, in real applications, there may have been restrictions on the number of mobile sensors due to the system cost.
