Among the challenges to copper wire bonding is the damage to bond pads that had been engineered for wire bonding with the softer gold wire.
In the present study, we report that a sustained depolarization slowly inactivated (half-time = 10 15 min) ROMK channels that had been engineered for increased affinity to internal polyamines.
The initial P450 had been engineered for maximal activity [ 23], meaning that it was not equilibrated to the more mild selection criterion of the experiments.
We began with a 21B3 peroxygenaseenase that had been engineered for highly efficient hydroxylation of 12-pNCA [ 23] (see Additional file 6).
The other 3 domains were variants of the wild-type domains that had been engineered for altered binding specificities towards trimethylated lysines: Cbx7 chromodomain A91K; JMJdoubleuble tudor domain D945K; and JMJD2A double tudor domain D945R.
The mutants had been engineered for other studies and differ from CBM4-2 by a set of 7 to 9 mutations (Table 1) mostly found in the binding cleft.
