Sentence examples for had average quality from inspiring English sources

Sequences that did not contain perfect matches to primer barcodes, were less than 200 bp in length, and or had average quality scores of less than 25 were discarded.

Based on those results, tools in the fastx toolkit were used to remove Illumina adapters, performing end trimming of reads, as well as filtering reads out of the dataset that had average quality values < 30 (sup. Figure  1).

Downloaded reads were subjected to the following cleaning steps: (1) read pairs were removed if either of the reads in the read pair had average quality score ≤ 32; (2) read 3' end nucleotides with quality scores < 10 were trimmed, and post-trim read pairs with either of the reads ≤ 72 bp were removed; and (3) reads with adaptor contamination were removed.

In particular, the last 10 bases have average quality score less than 20.

The 16S reads having average quality values <25 and possibly chimeric sequences represented 0.75 and 0.46% of the selected dataset, respectively.

As a simple filter, polymorphic loci with at least 6 alternative reads having average quality score > = 20 were identified as candidate SNPs; 2) Novoalign (http://www.novocraft.com) was used for alignment followed by application of SAMtools (http://samtools.sourceforge.net/) to identify candidate SNPs and indels.

Eucalyptus globoidea panels with densities higher than 800 kg/m3 had average bonding qualities lower than 3. Alternating E. globoidea and P. radiata veneers improved bond quality, and all samples passed the steam test, but only one sample passed the immersion test.

What our research shows is that if you've had average or high-quality sleep but are led to believe it was poor, you might see the same negative effects.

For both XauB and XauC all contigs larger than 1 kbp have average phred quality greater than 20, and nearly all (99% for XauB and 96% for XauC) these contigs have average phred quality greater than or equal to 40 (i.e. accuracy equal to or better than 1 error in 10,000 bp).

First, the 454-reads were removed if they were < 150 bp, had an average quality score < 35 in each 50-bp window rolling along the whole read, had an ambiguous base call (N), had any homopolymers of more than eight bases or did not contain the primer sequence; reads were then sorted by the tag sequences.

Before assembly, the reads were processed as follows: (1) reads of <60 bp or containing ≥1 N were discarded; (2) any read for which <25 of the first 35 bases had quality scores >30 was discarded; and (3) reads were trimmed to the first position for which a sliding 4-bp window had an average quality score of <20.