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The extraction with each reagent was carried out in duplicate after 16 h of end-to-end shaking.
After 1 h of end-over-end rotation at 4 °C, the lysate was cleared by high-speed centrifugation.
Cells were stained in the dark for 15 min at room temperature, and analyzed by flow cytometry within 1 h of ending the staining procedure.
The primary end point will be occurrence of nausea and/or vomiting within 36 h of the end of surgery.
One patient developed urticaria within 4 h of the end of the 2 mg/kg MRA infusion, lasting two weeks.
After 48 h of transfection, end joining efficiency was estimated by measuring luciferase expression that will only be achieved once plasmid is precisely rejoined in its circular form.
Because of resource depletion effects that accumulate over time in TX-TL reactions, especially after 1 2 h of incubation, end points of these fluorescence trajectories can give misleading quantifications of repression.
Plasma volume was measured (125I-dilution technique) at baseline, at 3 h after end of initiation of sepsis and at the end of the experiment 3 h later.
Sagopilone concentrations rapidly declined to ∼30% of peak concentrations within 0.5 h after end of the infusion.
These cells were generally isolated after 18 hpm that is ∼4 h after end of conjugation and consequently should be ∼4 h of KO.
Blood samples for gemcitabine analysis were collected in both treatment arms on day 1 and day 8 at the following time points: 0 h (immediately before start of infusion of gemcitabine), 0.5 h (at the end of infusion of gemcitabine) and 0.5 h, 1.5 h, 2.5 h, and 3.5 h after end of gemcitabine infusion.
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