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For the acetone control and acetone chemical spiking the following was performed: 25 gr of dry LUFA 2.2 soil was spiked with acetone (10 mL; CAS No. 67-64-1; purity ≥ 99.8%; supplied by Sigma Aldrich) or the appropriate organic-acetone solution for Phe, Car, PCA and DCA, equilibrated for 24 h, left to evaporate overnight, mixed with dry soil (75 g) and homogeneously moistened to 50% of the WHCmax.
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This solution was sonicated for 1 h and left to react for 18 h.
The sample was first annealed in air for 4 h, then left to cool down for 1 h and subsequently, transferred to the preheated vacuum furnace.
The digestion time were from 0 to 12 h (from left to right).
The reaction mixture was heated under refluxing temperature for 6 h, then left to cool at room temperature.
After addition of NBS was complete, the reaction mixture was refluxed with stirring for 2 3 h then left to cool to room temperature.
A mixture of thiazole 6 (2.86 g, 10 mmol) and DMF-DMA (1.19 g, 1.33 mL, 10 mmol) in DMF (3 mL) was heated on a water bath for 1 h, then left to cool to room temperature.
A mixture of 4-isothiocyanatobenzenesulfonamide 2 (2.14 g, 0.01 mol) and amines (0.012 mol) in dry dimethylformamide (15 ml) containing three drops of triethylamine was refluxed for 24 h, then left to cool.
The mixed solution was stirred with a magnetic stirrer at room temperature for 15 min, and was thermally decomposed in an oven under normal atmosphere at different temperatures of 600, 700, 800, and 900 °C for 6 h and left to cool down to room temperature before being ground to obtain LSMO nanoparticles.
Given that S phase itself lasts 20 25 min (Additional file 1), roughly 1 h is left to complete the cell cycle until the next S phase.
The resulting polyHIPE monolith was then Soxhlet washed in acetone for 24 h and left to air-dry overnight in a fume hood.
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