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The intestines were opened longitudinally, using a gut preparation apparatus, 28 washed in phosphate buffered saline (PBS) and fixed overnight in 10% neutral buffered formalin.
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Whole gut preparations were prepared identically, except that 25 guts were dissected and homogenized.
Gut preparations were examined under a phase contrast-fluorescent microscope at a wavelength of 360 440 nm to detect calcofluor-stained cellulose particles [22].
Unfortunately, 6 gave high fluorescence background in gut preparations and primary neurons that could not be washed out.
This could be a plausible reason for the greater efficacy of Fp.Cr in gut preparations of guinea-pig than in rabbit or mouse.
Additional bands that were detected in whole ant extracts in some instances were only found on thorax controls but never in gut preparations.
Based upon in vitro relaxant activity in isolated gut preparations, in vivo antidiarrheal activity was carried out in mice to confirm its antidiarrheal effect.
When tested on isolated gut preparations, the plant extract showed a concentration-dependent (0.01-10.0 mg/ml) spasmogenic effect in spontaneously contracting rabbit jejunum and guinea-pig ileum.
The spasmogenic effect was partially sensitive to atropine, while the presence of pyrilamine, SB203186 or hexamethonium had no effect in both gut preparations.
Recent evidence has also shown that enteric neuronal progenitor cells (ENPC) can be derived by autologous biopsy and can be grafted in organotypic gut preparations in vitro[ 9].
Mice were used in in-vivo prokinetic and laxative studies while in-vitro experiments were conducted on isolated tissues of rabbit and guinea-pig gut preparations suspended in a tissue bath to measure isotonic contractions.
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CEO of Professional Science Editing for Scientists @ prosciediting.com