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Thirdly, each embryo with a gut defect also displayed dorsal kinking of its tail (Fig. 4E and F).
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Ezrin−/− mice exhibit gut defects [4], raising the possibility that at least some of the defects we observe arise as a secondary consequence of nutritional stress.
Ihh is less widely expressed and mice deficient in Ihh are able to survive until late gestation but die due to skeletal and gut defects.
Determining nutrient utilization directly is important, especially in animals that suffer from gut defects and can provide meaningful information even when food intake appears normal.
Some models are also confounded by the presence of phenotypes not identified in patients, such as the existence of a severe gut motility defect [ 40, 41].
Tail defects and loss of movement/escape responses were scored from Stage 30 and gut coiling defects from Stage 40.
These include craniofacial anomalies, gut innervation defects and disorders of cardiac OFT septation, which occur in ∼5 per 1000 births (Dolk et al., 2010).
These defects were also noted at the lower MO1 dose of 2.5 ng, which did not generate the gut or tail defects, described below.
The formula could help scientists understand not just the variations of animal colourings but also more serious, related conditions called neurocristopathies that cause deafness, gut disorders, heart defects and cancer in humans when cells fail to move to the right positions.
Zoep mutants display strong gut and forebrain defects at 24 hpf [56], [57].
The gut and tail defects were found in <5% of embryos administered CTL MO, and CTL MO embryos never had motility defects (Fig. 5E).
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