Line 651 carries an inverted repeat of truncated GUS with a sequence homology of 566 bp in the inverted repeat region (Fig. 1b; [ 34]).
Replicating vectors derived from Potato virus X (PVX) and Tobacco rattle virus (TRV) were modified to contain the reporter gene β-glucuronidase (GUS) with a plant intron to prevent bacterial expression.
The pSK min35SGUS vector plasmid containing four copies of distinct REs: pathogenesis-related (PR1), salicylic acid responsive element (SARE), ethylene responsive element (ERE), or jasmonic acid responsive element (JAR) (sequences reported in Ref. 6) were selected for replacing GUS with a red fluorescent protein (RFP) reporter.
A typical annotation page contains the following information: Entry information: basic entry information such as protein name, accession number(s) (protein) and gene identifier (gi) with a clickable link to GenBank.
Rachel and Sam have vowed to replace Gus, named after Gus Poyet, with a new Spurs-inspired mutt.
In this vector, the gus gene with an intron is under the 35S promoter and the nptII gene under the nos promoter.
For designing a minimal length light-inducible Gacab promoter, the full-length Gacab P and the 5′ truncations of 197, 504, and 779 bp in length were fused with gus (uid A) gene and ligated into plant expression vectors.
His elimination of the brilliant Gus with the ringing of a detonator bell on a wheelchair was genius, and Walt, along with the rest of the audience, didn't really have time or inclination to think too much that the explosion was in a medical facility.
Meanwhile, Eva distracts Reenie's husband, Gus, with whom she forms a lasting connection in spite of being pulled apart by war-related travesties.
An hypothetical protein is also predicted (gi: 94403328) with an E-value of 4e-13.
