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It has been a blow to him that, just recently, Gus was found to have metastatic lung cancer, and has had to undergo chemotherapy.
AtTSPO-437:: GUS was found to be induced by 150 mM NaCl within 3 h of treatment, which is similar to qRT-PCR results of the endogenous gene.
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For the positive and negative controls, strong GUS activity was found in the P35S transgenic lines while no GUS activity was found in 1391Z.
Similar HS- and AZC-induced profile of GUS staining was found in Oshsp17.3p11/athsfA2/athsfA4a and Oshsp17.3p11/athsfA2/athsfA7a Arabidopsis plants (data not shown).
In mature plants at the 1-month-old stage, GUS activity was found in the vascular tissue, but not in mesophyll cells, epidermal cells or guard cells (Additional file 3).
The ligand-dependent GUS accumulation was found in all 15 independent transgenic lines examined.
In LR White-embedded tissue, GUS activity was found to be restricted to CCs, again irrespective of the presence of the 3'-UTR (Fig. 2C,2E).
This screening mode accounts for the high number of gene trap insertion events detected, since GUS expression was found in 72 of the 141 F2 plants (51%).
Strong GUS signals were observed in root cap and stele for GmPIN1b transgenic primary root and lateral root (Fig. 8a and 8b), GmPIN1c promoter showed a similar pattern of activity but weaker than GmPIN1b (Fig. 5c and 8d); while a much stronger GmPIN1e::GUS signal was found in stele than in root cap (Fig. 8e and 8f).
The protein undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase (ID gi |125717508) was found to interact with cell division protein FtsA; putative, phospho-N-acetylmuramoyl-pentapeptide-transferase, cell division protein FtsW; and putative, undecaprenyl diphospho (UDP -N-acetylmuramoyl-L-alanyl-D-glutamate synthetase.
The PSI-BLAST search using this bacterial protein identified statistically significant similarities to PAQR receptors and various bacterial hemolysins III (e.g. a human PAQR receptor (gi: 38018661) was found in the ninth iteration with an e-value of 1e-6 and a sequence identity of 13%).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com