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SHR frequencies were scored using SHR trap lines that allow quantitative evaluation of SHR by counting individual recombination events that restore the ß-glucuronidase gene (GUS), visible as blue spots after histological staining [14].
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The GUS-specific signal was very high in roots and stems, while leaf samples showed only visible GUS activities.
No visible GUS activity was noted for the control.
GUS staining was visible in vascular bundles, columella, placental tissue and seeds.
Under control conditions, POP2 was mainly expressed in roots since no GUS staining was visible in shoots (figure 7A) whereas a strong staining was present in roots (figures 7B, D and 7F).
GUS staining was not visible with the GmPRP2p-471 construct.
Furthermore, GUS staining was not visible in any organs of plants containing the GmPRP2p-471 construct.
At 3 and 5 dpi, expression was switched off in most of the feeding sites but GUS staining was still visible in the cells surrounding the syncytia.
In the AtATG18apro: GUS plants, the roots have no visible GUS activity under control conditions.
When viewing AtRNLp::GUS activity, GUS staining became visible already in stage I lateral root primordia, and strong expression remained detectable during later stages of organ development.
There was no visible enhancement of GUS from the PvUbi2 promoter constructs in tobacco following heat shock induction treatment for 60 minutes at 42°C.
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