Gus, it should be noted, is not just any scout, oh, no.
"I told Gus 10 years ago that he should be grooming new leadership, but he would have none of it," Carl Bloice, a former colleague, said in 1993.
— the question to be answered, Michael goes on, isn't whether he should be Gus's friend, which is never going to happen, you can't just undo years and years of being a dick, life just doesn't work like that, no, the question is how to manage the situation, so that Michael isn't suddenly the asshole; because, although being an asshole would be within Michael's rights — "Correct.
Regarding VIGS experimental design, at least one negative control VIGS construct containing a 250 400 nt fragment of a non-plant origin gene, such as the Aequorea victoria Green Fluorescent Protein gene (GFP; GenBank accession E17099) or the Escherichia coli β-glucuronidase gene (GUS; GenBank accession AF305918) should be included.
"Gus and Tony thought I should be the arranger for the forthcoming recording session for Space Oddity," Buckmaster says.
"We should be monitoring gusd instead," one Twitter user wrote after an instructor was arrested on charges of sexual abuse; the instructor pleaded not guilty.
However, it should be noted that GUS activity of a promoter driving the GUSsYFP1 fusion was lower than when the same promoter driving GUS alone was transactivated by an effector construct (data not shown), possibly because the fusion protein was less stable or produced.
There is also a place, it should be said, for lovable South Americans, such as Ossie Ardiles, Gus Poyet, and all things Brazilian.
Gus Baker, of the campaign group Intern Aware, who drafted the letter, said the companies involved should be "absolutely ashamed of what they're doing".
However, further investigation should be carried out to figure out the inconsistency of expression patterns between endogenous mRNA and the reporter GUS gene.
