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After the opening credits, the story returns to the present day, where Walt's partner Jesse Aaron Paull) has just shot Gale to death on orders from Walt, who insisted that if Gale is dead, Gus could not kill them as he had planned because he would have nobody to produce meth for him.
These results are in line with previous published literature showing that the genomic protein fusion of BB to GUS could not be induced by auxin.
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Unfortunately, GUS activity could not be determined in fruits, as the apple seedlings had not reached reproductive maturity during the experiment; however, tissue-specificity was determined by measuring GUS activity in roots.
GUS activity could not be detected in the roots; in the above-ground parts, the range of pPLAIIIδ expression was restricted in response to the lower IAA concentration treatments (1 and 10 μM), and the higher concentrations of exogenous IAA (100 and 1000 μM) suppressed the expression of pPLAIIIδ significantly.
The script was kicking around for almost seven years and even a director as accomplished as Gus van Sant could not find actors who were willing to undertake it.
From Mencius forward, fidelity to gi was often defined in terms of one's willingness to sacrifice one's life if realization of gi and one's continued existence could not be realized in tandem.
As expected, GUS activity did not change in 35S::GUS plants after treatment, showing that hormone treatments could not induce this promoter.
Since osrecql4-1 mutant has a GUS expression cassette in its T-DNA region, we could not evaluate homologous recombination frequency using the GU-US recombination substrate system.
We could not define a cell-specific pattern of GUS staining among the seven cells of the embryo sac, but GUS was unambiguously detected in the large central cell (Figure 3Bb).
GUS from the PvUbi1, PvUbi1+3 and PvUbi1+9 promoter constructs could not be visually observed in T1 seedlings at 10 and 17 days after germination, so these promoter variants were not studied in subsequent experiments (data not shown).
In cases where the program could not find an identifier, we used gi or RefSeq numbers to manually search co-identifiers in the UniParc [ 46], IPI [ 47], Entrez [ 48] or Ensembl [ 49] databases.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com