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The first aim of this approach was to objectively delineate which features of the chromatographs and gene expression profiles (GAPDH, B2M, GUS) could be used as predictors of the total RNA degradation as reflected by the degradation time.
If GUs of different sizes are assumed to be evenly distributed in each plant (see the following section), the surface on which new GUs could be deployed would increase notably less than the number of GUs.
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However, in the transgenic plants harboring its URS:: GUS cassette, the GUS activities could be detected not only in the floret husks but also in the vascular bundles of leaves and roots.
All Gus transgenic lines showed obvious blue coloration in enzyme activity tests, indicating that the Gus gene could be normally expressed in all the lines.
In five cases GUS activity could be observed in the roots (data not shown).
ARR5::GUS expression could be observed in the tip of filaments as noted earlier [ 63].
Transient GUS expression could be seen 5 days after infiltration with expression levels plateauing 10 days after infiltration.
GUS staining could be detected only in cells of the mature gametophyte that were likely present at the time of bombardment.
While GUS activity could be detected in several adjacent cells (Fig. 2C), this is most likely due to diffusion of either the GUS protein or reaction product from an individual cell transiently expressing the GUS gene.
Further examination showed that the faint GUS activity could be detected from the uni-nucleate stage of pollen and the strongest activity was observed at the mature stage of pollen.
In a long-term callus cultures of pearl millet (Pennisetum glaucum), a gradual decrease in GUS activity could be associated with increased methylation levels, 18 month after transformation [ 102].
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